Application of sodium dodecyl sulfate polyacrylamide gel electrophoresis to fractionation of urine
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High-temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis.
The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...
متن کاملSimplified sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unconcentrated urine with enhanced resolution and detection sensitivity.
We applied a simple sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to urine. The method, developed for serum protein analysis (Clin Chem 1984;30:475-9), has a high sample throughput and gives excellent resolution with unconcentrated urine. It clearly distinguishes and characterizes proteinuric urine (7.5 microL) by Coomassie Blue staining and gives complex silver-stained patte...
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We describe a procedure for the convenient separation of proteins by sodium dodecyl sulfate/polyacrylamide gel electrophoresis of urine from cases of renal disease. A precipitation method that requires no special apparatus was used to concentrate the urinary proteins; for electrophoretic separation we used a commercially supplied polyacrylamide/cellulose gel slab. This method seems to be valuab...
متن کاملUse of DNA ladders for reproducible protein fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantitative proteomics.
In proteomics, one-dimensional (1D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein fractionation prior to mass spectrometric analysis to enhance the dynamic range of analysis and to improve the identification of low-abundance proteins. Such protein prefractionation works well for quantitation strategies if the proteins are labeled prior to separa...
متن کاملPolyacrylamide gel electrophoresis of mycoplasma proteins in sodium dodecyl sulphate.
Electrophoresis of constituent proteins in polyacrylamide gels has become a useful aid in the identification of mycoplasmas. Razin & Rottem (1967) showed that mycoplasma proteins dissolved in phenol and acetic acid give reproducible, species-specific electrophoretic patterns when separated in the presence of acetic acid and urea. We encountered difficulties when using this technique. Mycoplasma...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1979
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.22.285